2/14/2023 0 Comments What is cccp setting![]() ![]() ![]() These results suggest a role for membrane potential in the secretion of periplasmic proteins. Processing can be restored in CCCP-treated cells and in valinomycin-treated spheroplasts by dilution of the treated cells in fresh medium. ![]() Valinomycin, a potassium ionophore, also inhibits processing of the leucine-specific binding protein in spheroplasts. The levels of CCCP that inhibit processing also produce significant decreases in the membrane potential. The processing of both the leucine-specific binding protein and a plasmid-coded beta-lactamase is inhibited by phenethyl alcohol and by the proton ionophore, carbonylcyanide m-chlorophenylhydrazone (CCCP). The leucine-specific binding protein of Escherichia coli is a periplasmic protein that is synthesized as a precursor and subsequently is processed during its secretion into the periplasmic space. ![]()
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